Induction of unscheduled DNA synthesis in rat hepatocytes following in vivo treatment with dinitrotoluene
Identifieur interne : 001E37 ( Main/Exploration ); précédent : 001E36; suivant : 001E38Induction of unscheduled DNA synthesis in rat hepatocytes following in vivo treatment with dinitrotoluene
Auteurs : Jon C. Mirsalis [États-Unis] ; Byron E. Butterworth [États-Unis]Source :
- Carcinogenesis [ 0143-3334 ] ; 1982.
Abstract
The purpose of this study was to examine the induction of unscheduled DNA synthesis (UDS) by the potent hepatocarcinogen technical grade dinitrotoluene (tgDNT; 76% 2, 4-DNT, 19% 2, 6-DNT) using the in vivo-in vitro hepatocyte DNA repair assay. Male Fischer-344 rats were treated by gavage and hepatocytes were isolated by liver perfusion and cultured with [3H]thymidine. UDS was measured by quantitative autoradiography as net grains/nucleus (NG); ≥5 NG was considered positive. Controls consistently had − 3 to − 6 NG. A dose-related increase in UDS was observed 12 h after treatment, with 200 mg/kg tgDNT producing 26 NG. A 50-fold increase in the number of cells in S-phase was observed at 48 h after treatment. This increase in S-phase cells could be suppressed in the presence of 10–20 mM hydroxyurea (HU), while the same levels of HU did not affect the level of UDS at 12 h after treatment. 2, 4-DNT produced only a weak response, in contrast to 2, 6-DNT which was a potent inducer of UDS. Treatment of female rats with tgDNT yielded only modest increases in UDS and DNA replication relative to males. These results are consistent with the carcinogenicity studies and indicate that tgDNT is a potent genotoxic agent, with 2, 6-DNT contributing the major portion of the effect.
Url:
DOI: 10.1093/carcin/3.3.241
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000707
- to stream Istex, to step Curation: 000674
- to stream Istex, to step Checkpoint: 000786
- to stream Main, to step Merge: 001E65
- to stream Main, to step Curation: 001E37
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Induction of unscheduled DNA synthesis in rat hepatocytes following in vivo treatment with dinitrotoluene</title><author><name sortKey="Mirsalis, Jon C" sort="Mirsalis, Jon C" uniqKey="Mirsalis J" first="Jon C." last="Mirsalis">Jon C. Mirsalis</name></author><author><name sortKey="Butterworth, Byron E" sort="Butterworth, Byron E" uniqKey="Butterworth B" first="Byron E." last="Butterworth">Byron E. Butterworth</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:91F2BDEFCDCDF672220F9D48EB2F24BE26DEA2E0</idno><date when="1982" year="1982">1982</date><idno type="doi">10.1093/carcin/3.3.241</idno><idno type="url">https://api.istex.fr/ark:/67375/HXZ-7X6PMMCT-Z/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000707</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000707</idno><idno type="wicri:Area/Istex/Curation">000674</idno><idno type="wicri:Area/Istex/Checkpoint">000786</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000786</idno><idno type="wicri:doubleKey">0143-3334:1982:Mirsalis J:induction:of:unscheduled</idno><idno type="wicri:Area/Main/Merge">001E65</idno><idno type="wicri:Area/Main/Curation">001E37</idno><idno type="wicri:Area/Main/Exploration">001E37</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Induction of unscheduled DNA synthesis in rat hepatocytes following <hi rend="italic">in vivo</hi> treatment with dinitrotoluene</title><author><name sortKey="Mirsalis, Jon C" sort="Mirsalis, Jon C" uniqKey="Mirsalis J" first="Jon C." last="Mirsalis">Jon C. Mirsalis</name><affiliation wicri:level="2"><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Chemical Industry Institute of Toxicology, Research Triangle Park, NC 27709</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation><affiliation wicri:level="2"><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>1Present address: SRI International, Building 205, 333 Ravenswood Avenue, Menlo Park, CA 94025</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Butterworth, Byron E" sort="Butterworth, Byron E" uniqKey="Butterworth B" first="Byron E." last="Butterworth">Byron E. Butterworth</name><affiliation wicri:level="2"><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Chemical Industry Institute of Toxicology, Research Triangle Park, NC 27709</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Carcinogenesis</title><idno type="eISSN">1460-2180</idno><idno type="ISSN">0143-3334</idno><imprint><publisher>Oxford University Press</publisher><date when="1982">1982</date><biblScope unit="vol">3</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="241">241</biblScope><biblScope unit="page" to="245">245</biblScope></imprint><idno type="ISSN">0143-3334</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0143-3334</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">The purpose of this study was to examine the induction of unscheduled DNA synthesis (UDS) by the potent hepatocarcinogen technical grade dinitrotoluene (tgDNT; 76% 2, 4-DNT, 19% 2, 6-DNT) using the in vivo-in vitro hepatocyte DNA repair assay. Male Fischer-344 rats were treated by gavage and hepatocytes were isolated by liver perfusion and cultured with [3H]thymidine. UDS was measured by quantitative autoradiography as net grains/nucleus (NG); ≥5 NG was considered positive. Controls consistently had − 3 to − 6 NG. A dose-related increase in UDS was observed 12 h after treatment, with 200 mg/kg tgDNT producing 26 NG. A 50-fold increase in the number of cells in S-phase was observed at 48 h after treatment. This increase in S-phase cells could be suppressed in the presence of 10–20 mM hydroxyurea (HU), while the same levels of HU did not affect the level of UDS at 12 h after treatment. 2, 4-DNT produced only a weak response, in contrast to 2, 6-DNT which was a potent inducer of UDS. Treatment of female rats with tgDNT yielded only modest increases in UDS and DNA replication relative to males. These results are consistent with the carcinogenicity studies and indicate that tgDNT is a potent genotoxic agent, with 2, 6-DNT contributing the major portion of the effect.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Caroline du Nord</li></region></list><tree><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Mirsalis, Jon C" sort="Mirsalis, Jon C" uniqKey="Mirsalis J" first="Jon C." last="Mirsalis">Jon C. Mirsalis</name></region><name sortKey="Butterworth, Byron E" sort="Butterworth, Byron E" uniqKey="Butterworth B" first="Byron E." last="Butterworth">Byron E. Butterworth</name><name sortKey="Mirsalis, Jon C" sort="Mirsalis, Jon C" uniqKey="Mirsalis J" first="Jon C." last="Mirsalis">Jon C. Mirsalis</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001E37 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001E37 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:91F2BDEFCDCDF672220F9D48EB2F24BE26DEA2E0
|texte= Induction of unscheduled DNA synthesis in rat hepatocytes following in vivo treatment with dinitrotoluene
}}
| This area was generated with Dilib version V0.6.33. |  |